The...Yeastiest way to produce energy?
February 28, 2001
Dr. Krufka MWF 1:30-3:30 Lab
Living organisms catabolize organic molecules within their cells and use the energy released to manufacture ATP by phosphorylating ADP. Many prokaryotes and virtually all Eukaryotes phosphorylate ADP either through fermentation (anaerobic) or respiration (aerobic). Both of these processes involve oxidation of foodstuffs, yet only the latter requires oxygen.
Got that? Didn’t think so. Cellular respiration is a very complex process that consists of many steps that take place inside the cell, in an organelle called a mitochondrion. Mitochondria are responsible for converting digested nutrients into the energy-yielding molecule adenosine triphosphate (ATP) to fuel the cell's activities. This function, known as aerobic respiration, is the reason mitochondria are frequently referred to as the powerhouse of the cell. There are two types of respiration that occur in cells to produce energy. When oxygen is available in the cell, aerobic respiration, as mentioned previously, occurs. When oxygen is not re
Temperature can change all forms of life. Certain cells, like yeast cells produce ethyl alcohol through fermentation. In fact, ninety percent of the energy yield from the respiratory breakdown of nutrients depends on the existence of aerobic conditions. We believed that the chemical reaction that occurs to produce carbon will occur more rapidly when at a high temperature, and slower when at a colder temperature. Each of the pipets were placed in very different temperatures. We used a mixture of 20 grams of yeast, mixed with 1 sucrose, a sugar, and 125 milliliters of water for our yeast mixture. Fermentation is the process by which the electrons and hydrogen ions from the NADH produced by glycolysis are donated to another organic molecule. The more CO2 produced in every five minute interval, the faster cellular respiration is taking place inside the cell. Respiration in the presence of oxygen makes possible the complete oxidation of nutrient compounds into carbon dioxide and water. adily available, the process of anaerobic respiration occurs. Aerobic respiration is highly favorable to humans and animals because we require large amounts of energy to function properly. After a half an hour of being emerged in their respective temperatures, we found that the yeast in the incubator, after being in a very warm temperature of 50 degrees Celsius, had the most dramatic CO2 production of the three tested. We then took readings of the carbon dioxide level in each pipet every five minutes. Using three glass pipets to hold the yeast mixture, we then sealed one end, and flipped the pipets upside down so to record the level of carbon dioxide that developed.