Protein Electrophoresis
Protein Electrophoresis Disagrees with Phylogenic Relationships in 5 Species of Fish Proteins are involved in nearly all aspects of life in eukaryotes, from structural support, to signaling within an organism, and everything in between (Campbell & Reece, 2002). Proteins originate in DNA in the form of nucleotide sequences, so testing proteins is another way of testing the genetic structure of organisms. Also, proteins are abundant in all animals, which make them excellent sources of data. We believe that different animals will contain different proteins within their muscles, but will have some similarities depending on how closely related the two animals are. These similarities allowed us to compare proteins in five species of fish-salmon, sturgeon, smelt, catfish, and trout. From the similarities in proteins, we derived the evolutionary relationships between these animals. These relationships are summarized in a graphical representation called a phylogeny. Phylogenies are designed to show evolutionary relationships based on molecular and/or morphological properties. The phylogeny on the next page was contained in the lab manual (Landel & McFarland, 2004). This phylogeny was the basis for our experiment,
Gel Samples and VolumesLane Sample Volume (μl)1 Actin / Myosin Standards 102 Salmon 103 Catfish 104 Trout 105 Sturgeon 106 Smelt 107 Kaleidoscope Standard 10Results: The electrophoresis of the gel gave decent results, but could have been better. Because the last two proteins were not visible, an assumption was made for an approximate location based on data found in the lab manual, in order to keep the continuity of the standard curve as well as for accuracy of the assumed molecular weights of the bands. 8 24Soybean trypsin inhibitor Orange 30. Gel Visualization 1 2 3 4 5 6 7kDa:13510050 Proteins:Myosin (Heavy)ActinTropomysinMyosin (Light) RangeLanes: 1. After electrophoresis, the gel was removed from the gel box and transferred gel to a container with 40 ml Bio-Safe Coomassie Blue Stain. If the same type of results are obtained a second time, it is a little more unlikely that the conflicts in the proposed phylogenies are due to experimental error. In an ideal experiment, all five samples would have exhibited only the known bands and thus make the results more clear.
Common topics in this essay:
Discussion DNA,
Aprotinin Blue,
Bio-Rad Laemmli,
Actin Myosin,
Campbell Reece,
Landel McFarland,
Smelt Salmon,
Kaleidoscope Standard,
Myosin Standards,
Myosin Blue,
salmon catfish,
trout sturgeon,
catfish trout,
kaleidoscope standard,
distance traveled,
standard curve,
landel mcfarland 2004,
landel mcfarland,
closely related,
molecular weights,
flip-top micro,
salmon catfish trout,
catfish trout sturgeon,
flip-top micro tube,
5 6 7,
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Acceptance Essays
