Abstract on a research article

. . .
These complexes are involved in the transport of TNFá mRNA from the nucleus of a cell to the cytoplasm. NZW mice are reported to be low producers of TNFá protein when stimulated with interferon ã (INFã) and lipopolysaccharide (LPS) which normally cause increased production of TNFá. A treated with LPS or INFã produced more TNFá protein than NZW mice, which hints towards the efficiency of TNFá protein production in B10. It has been demonstrated that NZW mice contain a trinucleotide (GAU) mutation in the main ARE of TNFá mRNA 3’-UTR, which affects the post-transcriptional regulation of TNFá production. binding region is present within the ARE, whereas the second is located 147 base pairs downstream of the first ARE. In order to confirm that the decrease in binding of macrophage proteins to the 3’-UTR of NZW TNFá mRNA is due to trinucleotide insertion in the main ARE, copies of the protein binding region containing insertional mutations and protein binding region with no mutations were made. RNA binding assays were performed with cRNA as probes. From electrophoreses it was shown that RNA-binding proteins bind less to mRNA containing the GAU trinucleotide insertion in the 3’ UTR, which in turn prohibits TNFá mRNA from being able to be effectively translated. The results showed macrophage complexes A, B, and C bind less to the 3’-UTR of TNFá mRNA that contain GAU insertional mutations in the ARE. The extracts were probed and ELISA analysis was performed with the different supernatants. RNase T1, which cleaves any mRNA not bound to macrophage proteins, was then employed in RNA-binding assay.

Common topics in this essay:
, LPS INFã, RNase T1, NZW TNFá, tnfá mrna, macrophage proteins, 3-utr tnfá mrna, binding macrophage proteins, nzw mice, tnfá protein, protein binding, 3-utr tnfá, binding region, binding macrophage, trinucleotide insertions, gau trinucleotide, rna binding assays, binding assays performed, trinucleotide insertions main,

Approximate Word count = 497
Approximate Pages = 2 (250 words per page double spaced)

Already a member? Click here