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This is a lab report on Drug Analysis-Thin Layer Chromatography

Objective: To detect the separation and tentative identification of components present in mixtures by using thin layer chromatography. Materials and Methods/Procedures: A thin layer plate was obtained and a baseline was drawn in pencil approximately 1.5 cm from the bottom of the plate. Seven equidistant spots were marked and labeled on the baseline. Six samples were obtained and using a new capillary tube for each sample, a spot of each drug solution was placed on the appropriate mark on the chromatography plate. The spots were allowed to dry, and one drop of the five drug samples were spotted on the mark labeled ‘mixture'. The mark labeled ‘unknown' was spotted with the unknown sample a couple of times. The plate was placed in a prepared ‘solvent chamber', and when the solvent moved up the plate ¾ of the way, the plate was removed and allowed to dry. The plate was then examined under short wavelength ultraviolet light. Results: After observing the plate, the drug present in the Unknown sample was identified as Phenacetine. The spots observed in the "Mixture" were compared to the spots of each individual drug and it was noted that the unknown #6, identified as Phenacetine, was the only spot to match with the number 7 mixture. Spot #1-caffeine, and spot #5-quinine were noted to be at the same level. Discussion: When using thin layer chromatography to observe spots of individual drugs it was noted that most of the drugs were not level or were a little off track from the others. Conclusion: Thin layer chromatography allowed the student to determine what the unknown drug was by running spots of the sample with known drug samples on the same plate, and then comparing the results. This also prevented any uncertainties which could have been caused by variations in the system....

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This is a lab report on Drug Analysis-Thin Layer Chromatography. (1969, December 31). In Retrieved 04:53, July 07, 2015, from