To Measure the Immune Response
To Measure the Immune Response, through Lysozyme Activity and Leucocyte Counts in Atlantic salmon infected with ADG, Neoparamoeba pemaquidensisNeoparamoeba pemaquidensis is an amoeboid protozoan that causes amoebic gill disease (AGD) in salmonids in several different countries (Mundy et., 2001). AGD is the main disease affecting the salmon industry in Australia (Powell & Clark 2004), and is the most important infectious disease affecting sea-caged salmon in Tasmania (Findlay & Munday 1998) it has been reported that pre-existing gill lesions and other amoebae may play a role in the pathogenesis of this disease (Munday et al. 1990), Little information is available on the epidemiology of AGD and the biology of Neoparamoeba pemaquidensis the aetiological organism of AGD. The disease appears as white mucoid patches on the surface of the gill lamellae, with pronounced mucous production (Mundy et al 1990). N. pemaquidensis has been associated with sever gill disease and significant mortalities among salmon reared in seawater (Bruno and Poppe, 1996). The immune response of a fish is strong and measurable (Kuby 1997). The non-specific, innate arm of the immune system exists to either prevent pathogens from entering
Five salmon were sampled, three controls and three infected 12 days post inoculation. The lysozyme activity in the kidneys was also 10 to 20 times greater than in the blood which is similar to this study. Therefore you have immune factors working during the innate response which may be the same factors used in an adaptive response (Nowak, 2003). 1 BackgroundThe experiment was conducted at the aquatic centre, University of Tasmania, Launceston and consisted of two treatments: (1) a control- no treatment and (2) the treatment of salmon infected with AGD. A standard curve was constructed using the concentrations of hen white lysozyme and the absorbances of the serial dilutions. Journal of Fish Diseases 21, 121-125. Possible explanations for the stagnated results may be due to no monitoring be conducted relative to time therefore, the development progress of the immune response could not be properly measured. Microplate absorbances were read using a spectrophotometer at 450 nm over six minute intervals. The evolution of the stress concept. Conversely, lysozyme concentration was significantly higher in the infected compared to the control (Fig- 2). 3 Lysozyme ActivityFive samples were taken from each fish for both control and treatment.
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