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How Temp affects rate of react

In this investigation I am trying to find out how the temperature of water affects the reaction rate between amylase and starch. The thing that I am going change are the temperature of water, which are 10 C, 20 C, 30 C, 40 C, 50 C. The things I will measure are the colour of the mixture in the present of iodine each minute until it stays a constant brown. I researched this information in a previous lesson in which we did the experiment at one temperature and timing the amount of amylase to break down the starch. Amylase, a carbohydrase found in saliva, works best in slightly alkaline conditions at a pH of about 7.5. I think this information applies to everyday life because it gives us the information about what temperature starch is used in our body.I predict that the 40C experiment will be the fastest to breakdown the starch into sugar. The scientific reasons why I think this might be is because 37C is our bodies temperature and analyse is a biological enzyme in our


Our body temp is around 37C so this is the best for the starch to work at. 33These results tell me that 30C - 40C was the quickest temperatures to make the starch breakdown. I will vary the temperature of the water. My conclusion disagrees with my prediction because I said that the higher the temp the faster the starch would be broken down. However, as I do not know how amylase will react at a higher temp and I know that any temp below around 40C works slower so I think that it will be faster at the highest temperature. I think this might be because I did measurements at every minute and if I done measurements at every half-minute they would be more accurate and show a better set of results. As you can see I have done two graphs. Evaluation:My results were not as accurate as they could be because the optimum temperature should have worked out to be 37C. 550C Black Drk Brown Tan Tan Tan 1/3 = 0. Equipment:200ml beakerTest TubeAmylaseStarchIodineSpotting tileStop ClockPipetteMeasuring CylinderWaterThermometerIceKettleDiagram:Step by Step:1) Collect Equipment2) Fill beaker with 200ml water at the specified temperature3) Put 5ml starch, 1ml amylase into test-tube and place in water4) Start the Stop clock5) While waiting for 1st minute fill spotting tile with iodine6) At the each minute interval fill one spot to see colour using pipette7) Repeat until colour does not change8) Try again for different temperatures9) Record results10) Pack away equipmentI will take measurements every minute. This shows that my results are not as accurate as they could be because the optimum temperature should be around 37C. 332nd set of results - Time (min) Temp 1 2 3 4 5 1 over t10C Black Black Black Black Black 1/5 = 0. 220C Black Drk Brown Drk Brown Brown Brown ¼ = 0. 540C Black Drk Brown Tan Tan Tan 1/3 = 0. The first one shows how long the starch and amylase took to react at each temp on both set of results but the second graph shows the rate of reaction using 1 over t.

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