biology

             Investigation on factors affecting the enzyme Amylase
             Aim: An investigation into the factors affecting the activity of the enzyme Amylase - Planning
             I predict that as the temperature increases, the speed of the reaction will increase. When a particular temperature is reached, I believe the rate of reaction will dramatically decrease. I believe this because most chemical reaction happens faster when the temperature is higher. At higher temperatures molecules mover around faster, which makes it easier for them to react together? Usually, a rise of 10 degrees celius will double the rate of reaction. This is true for enzymes up to about 40 degrees celius. However at 40 degrees celius the enzyme begins to be damaged, so the reaction slows. By 60 degrees celius, the enzyme is completely denatured. I predict that the same will happen the further away the pH is from pH 7. I believe this because the optimum temperature for most enzymes is about pH 7 therefore the further away from pH 7 (either more alkaline or more acidic) the less affective the enzyme.
             To investigate the factors affecting the activity of the enzyme Amylase, I will adapt a pilot experiment, which investigates if the enzyme amylase breaks down starch. The pilot experiment was:
             1. Pour amylase solution into a test tube to a depth of 2cm.
             2. Half fill another test tube with a 4% starch solution.
             3. With a pipette, place a drop of iodine into each dimple in a dimple tray.
             4. With a glass rod lift a drop of the starch solution from the test tube and mix it with the first drop of iodine in the first dimple in the tray. A blue/black colour should develop; this will be used as the control.
             6. Pour amylase solution into the test tube of starch and shake quickly.
             7. Repeat steps 4 & 5 (for the amylase & starch solution mixture) every 30 seconds until a blue/black colour no longer develops.
             8. When there is no further change in the colour of the iodine, take ...

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