biology
Investigation on factors affecting the enzyme Amylase Aim: An investigation into the factors affecting the activity of the enzyme Amylase - PlanningI predict that as the temperature increases, the speed of the reaction will increase. When a particular temperature is reached, I believe the rate of reaction will dramatically decrease. I believe this because most chemical reaction happens faster when the temperature is higher. At higher temperatures molecules mover around faster, which makes it easier for them to react together? Usually, a rise of 10 degrees celius will double the rate of reaction. This is true for enzymes up to about 40 degrees celius. However at 40 degrees celius the enzyme begins to be damaged, so the reaction slows. By 60 degrees celius, the enzyme is completely denatured. I predict that the same will happen the further away the pH is from pH 7. I believe this because the optimum temperature for most enzymes is about pH 7 therefore the further away from pH 7 (either more alkaline or more acidic) the less affective the enzyme.To investigate the factors affecting the activity of the enzyme Amylase, I will adapt a pilot experiment, which investigates if the enzyme amylase break
Add 2cm3 of water to starch solution (see below). With a pipette, place a drop of iodine into each dimple in a dimple tray. I believe that all the results were skewed because the enzyme was not given enough time at each particular temperature to be fully affected before it was added to the starch. This will ensure that there are no abnormal results and it will increase accuracy. This includes a 'dent', which is called the active site. High temperatures make enzymes inactive: this is because they are proteins, which are damaged by temperatures above about 40 degrees celius. Repeat steps 4 & 5 (for the amylase & starch solution mixture) every 30 seconds until a blue/black colour no longer develops. Variables:I have chosen to repeat the experiment 3 times because it therefore allows me to calculate an average time. This is also because they are proteins, which are damaged by very acidic or very alkaline conditions. With a glass rod lift a drop of the starch solution from the test tube and mix it with the first drop of iodine in the first dimple in the tray. Amylase speeds up the breakdown of long chain starch molecules into smaller chains of maltose. I could not find a linear relationship between the temperature and rate of reaction, however with enough results (from a very large range of temperatures) a parabola could be made on Graph 1, it would then be possible to find a mathematical formula for the construction of the parabola and hence find the relationship between the temperature and rate of reaction. It should also be accurate enough for me to predict an optimum operating temperature to an accuracy of 5 degrees celius.
Common topics in this essay:
Adapted Experiment,
Enzymes Substances,
Evaluating Evidence,
Note Optimum,
Pilot Experiment,
Planning Prediction,
degrees celius,
test tube,
starch solution,
rate reaction,
enzyme amylase,
solution test tube,
solution test,
ph 7,
Amylase Aim,
40 degrees celius,
active site,
40 degrees,
10 degrees,
10 degrees celius,
amylase digest starch,
pour amylase solution,
,
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Acceptance Essays
