Enzyme Action Testing Catalase Activity
Enzyme Action: Testing Catalase ActivityEnzymes are proteins that serve as catalysts and accelerate chemical reactions in living organisms. Enzymes accomplish this by lowering the energy of activation of the organism it is acting upon, however enzymes will only lower the energy of activation for specific organisms, reducing chaotic chemical reactions. The reaction is carried out in it's entirety in the active site of the enzyme. The substrate (organism acted upon), binds itself into the active site, of the enzyme, and the chemical process begins. The enzyme's active site will reform itself for the substrate in order to form a perfect bond, this is called induced fit. This is only one of the changes that could occur to accommodate the substrate. For example, the enzyme might change the pH levels to promote the reaction. There are numerous factors that can affect the rate of the chemical changes, four have the greatest affect. They are the concentration of the enzyme, the temperature, the pH level, and the salt concentration. The higher the concentration of the enzyme the faster the rate of change there is upon the organism. The temperature affects the rate in this way; each enzyme has a specific
Then the gas pressure was measured inside the test tube as before. For the time being, however it is safe to assume a steady increase in the rate of reaction with respect to an increase in concentration. Four test tubes were labeled one to four respectively, and 3ml of 3% concentration H202 was added to each. There are very few factors that influence the function of the enzyme. The results of this experiment can be summarized as follows: This enzyme like others increases the rate of chemical change with an increase of concentration. In Test Tube (Degrees Celsius) Rate of Reaction (atm/min)0-5 3. In this experiment, some of theses factors will be tested, concentration, heat, and pH levels. This experiment should show what the optimum concentration of the enzyme and pH level, and temp that is needed for the enzyme to perform at it's peak rate. However too high of a temperature produces the same results. Table 1 Enzyme Concentration 1 drop 2 drops 3 drops 4 drops Rate of Reaction (atm/min) 0. Third, the pH level will affect the rate, H+ and OH- ions can cause the organism to change shape and become deformed, denature, and cause abnormal functionality. Table 2 Temperature Range (Degrees Celsius) Actual Temp.
Common topics in this essay:
H+ OH-,
Introduction Enzymes,
Rate Reaction,
Celsius Finally,
Reaction Rate,
test tube,
Results Data,
Enzyme Concentration,
rate reaction,
test tubes,
active site,
ph level,
gas pressure,
drops enzyme,
added test,
pressure measured,
concentration enzyme,
gas pressure measured,
summarizes rate reaction,
3ml 3% concentration,
inside test tube,
drops enzyme solution,
Degrees Celsius,
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