immunology grant proposal
I will be addressing the possible immunological barriers that may be involved with challengeto infection of Toxoplasmosa gondii, the protozoan that causes toxoplasmosis. It is widely knownthat toxoplasmosis is a devastating disease, with often drastic consequences upon infection. Inpregnant mothers, these consequences can be very horrid. Such effects are more felt by the unbornfetus than by the mother. These effects include abortion, premature birth, and severe growthretardation (Creasy et. al., 1994). Falkner et. al. have shown that the human fetus is capable of producing antibodies as early as ten weeks gestation time. We also know that antibodies totoxoplasmosis exist, which are of Ig G, Ig M, and, recently demonstrated, although in low levels, Ig These facts give rise to some questions regarding the bizarre reactions of the fetus, whichshould be more than protected enough from infection. Why is the fetus damaged, or even abortedfrom such a challenge, when it has the ability to produce antibodies? Are any of the antibodies
Determining the isotype is done bybreaking down the fragments and subjecting them to Southern Blotting, and the results of the blotwill tell if the fragments are the same. After establishing the presence of antibodies to toxoplasmosis in the fetus, assays todetermine the functioning of the system would be needed. This assay would call for the fetus to be infected with labeled toxoplasmosis directly, andsamples of blood to be drawn from the fetus after sufficient time has been allowed for any Ig-Agreactions to occur. BACKGROUND AND SIGNIFICANCE I will be addressing the fetal response to challenge with toxoplasmosis infection. If label is found in solution, itis safe to say that the antibodies, when in contact with antigen, can elicit a cell-mediated response. Some experiments can be conducted in vitro. The pregnant woman, placenta, fetus, andinfectious agents. The formation of plasma cells tells usthat the fetus can form memory to such an antigenic challenge, which is of importance if futureencounter occurs. The Fc fragments are of interest, because it is this segmentthat holds the key to answering if the 2 are of the same origin. (recv'd via Pub Med)9) Rugh, Rober. It is this form that can possibly reside in theplacenta, and thus release its contents into the blood and then cross the barrier to the fetus.
Common topics in this essay:
Southern Blot,
EXPERIMENTAL DESIGN,
BACKGROUND SIGNIFICANCE,
Ig Ig,
Repici Immunology,
Southern Blotting,
AJ Kourtis,
specific toxoplasmosis,
Co Philadelphia,
Immunology Vol,
EA Toxoplasmosis,
antibodies toxoplasmosis,
fetal serum,
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immune response,
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et al,
complement system,
toxoplasmosis antigen,
ig molecules,
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ig specific toxoplasmosis,
ig found fetal,
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Acceptance Essays
