osmosis
Osmosis is the diffusion of water across a selectively permeable membrane in living organisms from a hypotonic to a hypertonic solution. (Raven & Johnson, 2000) The speed of which one substance diffuses from one area to another is based upon the free-energy gradient between the two areas. For example, if two substances differ greatly between their areas, it allows the gradient between them to be steep which results in rapid diffusion. If, however, the gradient between the two substances is equal there is no diffusion and the net movement stops. Osmosis follows the same laws as those of diffusion but it will always refer to water as the principal solvent in cells. Because water is the universal solvent in cells, its diffusion across selectively permeable membranes is critical to living organisms. (Gibson-Brown Lab Notes, 2000) Osmosis can be simulated by using dialysis tubing to form bags to represent cells under different conditions and measuring the direction and rate of osmosis. The following experiment is to use the bags to measure the rates of osmosis in each bag. Each bag in the experiment is a model of a cell. The experiment requires the usag
Total weight changes in response to the difference in the independent variable; total weight is the dependent variable. Instead of knowing to replace the bag with a new one, I emptied as much as possible and refilled it with new solution. So the beginning weight may be altered due to the fact at which some of the previous solution was still intact and causing the bag to weigh more than it should. Clamp the open ends securely, rinse, and check for leaks. During the experiment an error arose with the contents in Bag A. Bag B contained 10ml of 1% sucrose that was placed into a large bowl that also contained 1% solution of sucrose. Place bag A in an empty beaker with just enough 50% sucrose to cover the bag. It is now where the procedure begins. (Gibson-Brown Lecture notes, 2000). The bags will all be filled with a pre-measured solution for each of them and set into containers containing the same solution at a different concentration level.
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