This lab was done to determine the relationship of gas production to respiration rate. Justin Pyka and I did the lab on December 12th, 2000 in Mr. Myers’ room. The lab was done with dormant pea seeds and germinating pea seeds. It was done to test the effect of temperature on the rate of cellular respiration in ungerminated versus germinating seeds. We had to determine the change in gas volume in respirometers. This was done to determine how much oxygen was consumed during the experiment. The respirometers contained either germinating, or non-germinating pea seeds. I think that the germinating seeds will have a higher oxygen consumption rate in a room temperature water bath than the non-germinating seeds. My reason for this hypothesis is that a dormant seed would not have to go through respiration because it is not a plant yet. A germinating seed would consume more oxygen because it is growing, and therefore would need to consume oxygen by going through the process of!
Methods and Materials
1 mL Glass Pipette Tubes
264 Germinating Pea Seeds
264 Dried Pea Seeds
100 mL Graduated Cylinder
25 Degree C Distilled Water Bath
10 Degree C Distilled Water Bath
Red Indicator (Safrin)
Syringe With Needle on End
See lab handout for methods of completing the experiment and recording the results.
Note: Two tests were done for each step.
See graphs for interpretation of results.
In the room temperature water bath, the glass beads, and the dry pea seeds and glass beads consumed the least amount of oxygen (see chart 1). The germinating pea seeds consumed the most oxygen. They consumed almost three times as much oxygen as the glass beads alone, and the glass beads and dry pea seeds.
According to my results, the vials containing the glass beads in the 10-degree ...