lab
If a laboratory assistant prepared solutions of 0.8M, 0.6M, 0.4M, and 0.2Msucrose but forgot to label them there would be no need for him to panic. Knowing theprinciples of water movement through the processes of osmosis and diffusion, one canconduct a rather simple experiment to determine which flask contains which solution. According to the principles of osmosis, water typically moves from solutions of low soluteconcentration to areas of high solute concentration. This information would allow one toform the basis of his or her experiment in trying to determine the concentrations of theunlabeled solutions. The specifics of the experiment will be discussed along with theresults one would expect from such an experiment. However, it is also important to knowwhy these predictable results would occur. They will also be discussed. In conducting the experiment it would be necessary to obtain dialysis tubing. Thiswould play the role of the semi-permeable membrane. This is important because without asemi-permeable membrane substances other than water, such as glucose, could exit theapparatus and throw off the results. Diffusion of the solutes would occur. Diffusion is the
Somesolutions would gain more mass while others would gain very little. 8M bag would gain the most mass in water while the other bags would gradually gainless water. It is also an important fact that the more solute the solution contains, themore water it will bring in. Each bag contains a higher solute concentration than the beaker they weresubmerged in. In this particular experiment,four bags would be made (taking solution from each of the four flasks). He would also be able to determine theothers by simply seeing which bags gained the next most mass and so on. The key is how much water wouldenter each bag. Knowing this the assistant would know that the bag thatgained the most mass would be the 0. According to the laws of osmosis water alwaysflows from areas of low solute concentration to areas of high solute concentration in orderto maintain equilibrium. Space would be left for expansion of the bag. The bags of the unknown sucrose concentrations would be left in thebeakers for about a half hour then removed and weighed again. This is further explained through hypotonic and hypertonic solutions. Therefore hypotonic means low solute, while hypertonic means highsolute.
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