In this investigation gel-electrophoresis is used in determining a killer’s identity. DNA found under the victim’s fingernails is compared to two possible suspects. The results ruled out Suspect 2 and victim himself. The bands from Suspect 1 matched the evidence taken from the fingernails.
Sarah Jeffries and Jim Candell had gone to a movie late in the evening and he needed to get some money from the ATM. Sarah stayed in the car while Jim got out to make the withdrawal. A man snuck up behind him and beat him to death with a tire iron. Sarah could not make his face out, but she did see his nose when he took his mask off. She said he had a Roman nose and possible scratch marks because Jim struggled with him. The police narrowed the suspects down to two and brought them in for DNA testing. This is a process of DNA testing is called gel-electrophoresis. DNA samples were taken from under Jim's fingernails. Then DNA was ta
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The reason DNA was taken from the victim was to make sure the DNA under his fingernails wasn't his own DNA. The agarose was placed in a microwave until melted. Then the gel was left alone for the time listed above. Then the evidence DNA was placed in the second well. 10 ml of buffer solution was poured into the gel box to barely cover the surface and surround the comb. 10 ml of molten agarose is poured evenly into the gel box to fill the central cavity and wrap around the wells. After 24 hours the charge was turned off staining solution was placed on the surface of the gel. Suspect 1’s bands matched the spacious bands of the evidence, unlike suspect 2.
Results
The bands appeared in rows under the wells.
Materials and Methods
First the gel was made from agarose. A lid was then placed on with positive and negative wires running from it to a battery and turned on. The DNA tubes were then placed in a centrifuge to spin the liquid to the bottom.
Approximate Word count =
647
Approximate Pages =
3 (250 words per page double spaced)
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