were obtained and labeled. There were five fish samples (Table 1): a smelt, a sturgeon, a trout, a catfish, and a salmon. A small piece of each fish muscle was cut and transferred into a labeled flip-top micro tube. The labeled flip-top micro tubes were closed and flicked 15 times. They were incubated for 5 minutes at room temperature. Each sample mixture was transferred from each flip-top micro tube into a labeled screw-cap micro tube. The Kaleidoscope pre-stained standards (KS) and the actin and myosin standards (AM) were obtained. The AM and fish samples were heated for 5 minutes at 95∘C. KS, AM, and fish samples were loaded into wells on the gel. The electrophoresis gel box was turned on for 30 minutes at 200 V. After electrophoresis, the gel was removed from the gel box and transferred gel to a container with 40 ml Bio-Safe Coomassie Blue Stain. The stain solution was discarded. The gel was de-stained in a large volume of water for 30 minutes. The gel was dried using GelAir cellophane. The full method can be found in Landel and McFarland (2004) and Bio-Rad (2000).
             Table 1. Gel Samples and Volumes
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