corded at high recording speed.
             To measure the dose response relation for Ca2+, the muscle was stimulated at 0.4Hz, with the solution containing a starting amount of 0.2 mmolL-1 of Ca2+ , a single twitch was captured at steady state, successive amounts of 0.4 mmolL-1 of Ca2+ were added to the solution and steady state force of a single twitch was captured at each new added amount.
             After flushing the organ bath a few times, an aliquot containing ouabain was used to determine its effect on the rat cardiac muscle at 0.4Hz. Steady state force measurements were taken at 0.25 ml of the aliquot but no change was noticed, so we double the amount and measured steady state, again no change in steady state was noticed, finally a total amount of 1.25 ml was added to measure a noticeable force response.
             After flushing the organ bath a few times, 0.05ml aliquot that contains isoprenaline was added to the solution; no change of force or duration was noticed. Then a total of 0.2 ml of the aliquot (containing 2.0 ìmolL-1 of isoprenaline) was added to see a noticeable change in the force response.
             A 0.4Hz stimulus was applied to the muscle at control conditions then, the bath was filled with the provided caffeine solution (containing 10 mmolL-1 caffeine and 1.5 mmolL-1 Ca2+). A single stimulus was recorded at that frequency then another using a 1s burst stimuli of 2Hz (the suggested 10 Hz stimuli did not show any noticeable effect, the cell was fatigued)
             • The 50Hz noise from the main power supply was being recorded in chart, made it more difficult to analyse, we should have repeated every part of the experiment many times and averaged them out to reduce the signal to noise ratio, but we didn't due to time limitations.
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