he maximal velocity of the reaction. These two values can be determined from the double reciprocal of the Michalelis-Menton equation or the Lineweaver-Burke Plot, with the y intercept being 1/ Vmax, and the x intercept being
-1/ Km. the equations are as follows:
Michalelis-Menton velocity of reaction= Vmax (substrate concentration)/( Km's)
Lineweaver-Burke plot 1/velocity= Km/ Vmax*1/sibstrate concentration+1/ Vmax
Methods: Part 1: Effect of Substrate Concentration on Velocity
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