DNA, Deoxyribonucleic acid, is a double stranded, helical nucleic
acid molecule which determines inherited structure of a protein. The
"steps" are made of bases: adenine, guanine, cytosine, and thymine. The
sides are sugar and phosphate molecules. Restriction enzymes are
enzymes that cut DNA at restriction sites, leaving fragments blunt or
sticky. The restriction fragments are separated using a technique called
DNA has a negative charge so when an electrical charge is
applied it makes DNA move to the positive side. DNA is placed in
agarose gel. Smaller fragments move faster. The purpose of this lab is to
separate DNA fragments using gel electrophoresis. Hind III cuts AAGCTT
between the two irst A's. EcoRI cuts at GAATTC between the G and the
A. Hind III and EcoRI both make sticky ends.
Our results for this lab were EcoRI separated into five fragments.
Hind III separated into four fragments. The control only had one fragment.
(See chart A and figure 1-1 for distances)
The purpose of this lab was to see how gel electrophoresis
separates DNA fragments. We used Hind III, EcoRI, and a controlled
enzyme. Some fragments were hard to see because of smearing. These
were the bigger fragments. Loading the DNA was difficult and if you
weren't careful you could rupture the wells which ruined the lab. We,
fortunately, did not run into this problem.