take a funnel with glass wool in it, I chose glass wool rather than filter paper because the catalase could have been adsorbed by the filter paper. Then I will add 5ml of the enzyme catalase to the conical flask and for the substrate concentration of 10% I will add 2ml of Hydrogen Peroxide and 18ml of water (18+2= 20, I will always use 20ml) every time I when I will increase the concentration by 10% I will increase the H2O2 by 2ml and decrease the H2O by 2ml. I will time how long it takes to produce 20ml of gas in the gas syringe. I chose the gas syringe rather than to count the bubbles produced in a measuring cylinder because it is easier to use, the results will be more accurate and the gas syringe reduces the possibility of gas escape. I will tabulate my results and highlight them in some way so they are visible I will interpret my results in to a line graph. I will also added a line of best fit to the results on the graph and with the results I get I will work out the ½ Vmax.
             Here is a blank copy of my results table, which I fill in later when I get my results.
             To make my experiment a fair test I need to ensure that all the variables must be kept the same for all the experiments except for the concentration of Hydrogen Peroxide.
             I will accurately measure out the Hydrogen Peroxide and enzyme solution using a pipette and measuring cylinder. I will use glass wool rather than filter paper because if I use filter paper then the catalase could be adsorbed by the filter paper, which will no longer make my experiment a fair test. I will time how long it takes to produce 20ml of gas by using a stopwatch accurately. For each concentration I will make sure that there is no excess catalase or substrate in the measuring cylinders I use by cleaning them. I will hold the rubber bung connecting the conical flask and the gas syringe so it does not open and let out any gas.
             I predict that the more the concentration of substrate ...