Instruction Manual
The purification process:
Recrystallization & Filtration
Jason Williams
09-13-00
NATS 4310
Table of Contents
Preface
Chapter 1 Equipment involved and preparation
Glassware and accessories
Proper sterilization of equipment
Safety precautions
Chapter 2 Setup procedure before purification
Equipment setup
Choosing of solvent
Chapter 3 Gathering of an unknown compound and the first step: filtration
Collection
Filtration
Repeat Filtration and Cleaning
Collection and measuring of crude sample
Chapter 4 Recrystallization process
Solvent addition
Induction of Crystals
Collection
Chapter 5 Evaluation methods for purity
Small scale test
More accurate tests
Chapter 6 Frequently asked Questions and trouble shooting
Index
Preface
To begin, the intent of this booklet is to give instructional advice. The instructions given will provide a basis to allow an individual to perform the necessary task to undergo the purification process. The design of the manual is to instruct students in a college laboratory setting. In particular, the area of science that the manual will focus on is Organic Chemistry. Students will be able to refer to this instruction guide throughout the semester because this process will be put in use often. The purification process if an essential part of chemistry, and the student must recognize its importance. Without a purified product, many of the drugs on the market would not be on sale. Even though these purification procedures are on a small scale, it will provide a starting point for those who are just beginning Organic Chemistry. The skills needed to purify a product will come with practice and help from the instructor, but this manual will give the initial directions to help the student on purification. Once mastered, more techniques will help the student to gain a product that is pure enough to put on the market.
Chapter 1
The E...