Table 1 shows the conditions of this IEF electrophoresis. 15 ƒÊl of each cytochrome C, myoglobin, hemoglobin, and serum albumin were loaded into the well as indicated in Table 1. Well 1 is the bottom well in Figure 1. A voltage of 175 V and a current 79 mA was applied in the buffer of pH 8.6 for 50 minutes, and bubbles were observed on the electrode during the electrophoresis. Figure 1 shows that cytochrome (*1 in Figure 1) moved closest to the cathode, and serum albumin (*4) moved closest to the anode. Myoglobin (*2) and hemoglobin (*3) moved toward the anode, bu
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