ab manual. First 10ul of pBLU plasmid with the lacZ gene and ampicillin resistance will be added to 250ul of E. coli cells and left on ice for 20 minutes. Next 10ul of nutrient broth will be added to 250ul of E. coli and placed on ice for 20 minutes. The nutrient broth is a combination of sugars, minerals and amino acids. After the 20 minutes is up the tubes will be placed in a 42°C water bath for 90 seconds. The temperature shock aids the E. coli in transforming with the plasmid. After the 90 seconds the tubes are put back on ice. Next 0.5ml of nutrient broth are added to both tubes and placed in a 37°C water bath for
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