ng the CaM-kinase-II promoter gene. After insertion was complete, Tsien's team observed the new transgenic mice, now named Doogie, along with other wild-type mice. Compared to the wild-type mice, the Doogies "showed normal growth and body weights and mate normally. Their regular field behavior is indistinguishable from the wild-type mice (Ya-Ping 1999)." Tsien then used northern blot analysis, which is used to detect a specific RNA molecule from a mixture of RNAs fractionated on a gel to show the NR2B transgene was mainly located
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