ow water potential, is referred to as being hypertonic, whilst the less concentrated solution is hypotonic.
The concentration of solute particles is described as a molarity. One mole of any substance is the mass of 6.02 x 1023 particles of the substance. The molarity of a solution can be calculated using the below equation:
If a plant was exposed to a waterlogged environment, with the external solute concentration to the cell being hypotonic to the vacuole contents, the cell will not continue to take in water by osmosis forever. The cellulose wall provides a rigid barrier to uncontrolled expansion. A cell that is full of water is called turgid and cannot expand further as the inward force of the starched wall balances the outward pressure on the cell contents. This wall pressure is called turgor pressure and the internal outward force on the wall is called the osmotic pressure.
At the other extreme, a cell placed in a solution that is hypertonic to its contents will lose water molecules by osmosis. The cytoplasm will cease to exert a pressure on the cellulose cell wall and the cell, described as flaccid, will lack support. Water loss can continue to such an extent that the cytoplasm, and attached cell membrane, contracts and detaches from the cell wall. A cell in this condition is said to be plasmolysed and this damage is irreversible.
· Use care when working with glassware.
· Wash your hands before and after the lab.
· Use care when working around electrical light sources.
· Use care when using any chemicals in the lab.
· Care will be taken when using the scalpel.
· All laboratory surfaces will be kept clear and clean throughout the experiment.
The independent variable of this experiment is the molarity of the sodium chloride solution. This will be changed and should cause changes in the dependant variables. The molarity of the solution was chosen, as it will be relatively...